An Optimization Study for Chitosan Nanoparticles: Synthesis and Characterization

Abstract

Studies have been carried out to determine the optimum conditions for chitosan nanoparticles. Variousformulations have been made which can affect the size and polydispersity index of the nanoparticles andthe process variables have been investigated. These formulation and process variables were defined andoptimized to obtain the smallest particle size. The concentration of chitosan polymer and crosslinkerconcentration were studied as formulation variables. Agitation speed, agitation time, pH, light effect,sonication time and sonication power parameters were selected as process variables. In the experimentsperformed for one parameter, all other parameters were kept constant. The optimum conditions weredetermined by the effect of formulation and process variables on particle size and polydispersity index.For the characterization of chitosan nanoparticles, Zeta-Sizer, UV-Vis, FTIR and SEM analyticaltechniques were used. Optimum conditions for process variables were provided by adjusting the sonicatorat 50 W power value for 5 minutes and 30-10 pulse interval; while the formulation variables were foundto be chitosan:TPP mass ratio of 5:1, pH value of 5 and under light. After the optimum conditionsobtained BSA loading was performed and characterization studies were carried out. It was believed thatchitosan nanoparticles produced by optimum conditions determined as a result of the study can be used inmany areas including as a drug delivery system in future studies.